微图案产品

CYTOO 已经获得了针对各种细胞类型（原代细胞、干细胞、永生化细胞系）和研究领域设计和生产非常合适的微图案以及开发更多生理相关细胞模型和检测的全面专业知识。

我们生产 2 种形式的微图案产品：用于基础研究和活细胞成像的 CYTOO 芯片（与专用配件结合使用）和用于高内涵筛选的 96 孔 CYTOO 板（正在开发 384 个）。

每种细胞类型、研究领域和读数都需要微图案形状、尺寸、密度和涂层蛋白的特定组合，以实现z+使用。在本节中，您将找到具有预先设计的微图案的标准芯片和板，适合z常见的用途

（已发布的数据和内部数据）。入门产品旨在同时探索多种微图案形状和尺寸。Arena CYTOO芯片是不同尺寸的圆盘。筛选 Starter 或 Arena 产品的结果是双重的：识别标准产品中存在的

工作微图案设计，或者选择在设计定制产品时仍可以优化的微图案参数（尺寸、密度……）。

我们的所有产品都可选择纤连蛋白、荧光标记纤连蛋白或即用涂层（激活）微图案，以实现z+细胞粘附。

产品列表

部分文献列表

Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis

Hurtado L / Caballero C / Gavilan MP / Cardenas J / Bornens M / Rios RM

keywords:

pericentrosomal golgi ribbon organization, akap450 n-terminal fragments, microtubule nucleation, centrosomal activity, golgi apparatus positioning, directional cell migration, ciliogenesis, y-shaped micropatterned coverslips, cell movement

J. Cell Biol. 2011;193(5):917–933.

Spatial organization of the extracellular matrix regulates cell-cell junction positioning

Tseng Q / Duchemin-Pelletier E / Deshiere A

keywords:

intercellular junctions, cell line, extracellular matrix

Proc. Natl. Acad. Sci. U.S.A. 2012;109(5):1506–1511.

A contractile and counterbalancing adhesion system controls the 3D shape of crawling cells

Burnette DT / Shao L / Ott C / Pasapera AM / Fischer RS / Baird MA / Der Loughian C / Delanoe-Ayari H / Paszek MJ / Davidson MW / Betzig E / Lippincott-Schwartz J

keywords:

live-cell microscopy, contractility, 3d structures

J Cell Biol. 2014 Apr 14;205(1):83-96. doi: 10.1083/jcb.201311104. Epub 2014 Apr 7.

A Mechanical Checkpoint Controls Multicellular Growth through YAP/TAZ Regulation by Actin-Processing Factors

Aragona M / Panciera T / Manfrin A

keywords:

yap/taz, oncology, cell shape, cell proliferation

Cell. 2013; Aug 15 [epub ahead of print].

A mechanical coupling between trans‐synaptic N‐cadherin adhesions and the actin flow stabilizes dendritic spines

Chazeau A / Garcia M / Czöndör K / Perrais D / Tessier B / Giannone G / Thoumine O

keywords:

neuro, n-cadherin, filopodia, hippocampal, spines

Mol Biol Cell. 2015 Mar 1;26(5):859-73. doi: 10.1091/mbc.E14-06-1086. Epub 2015 Jan 7.

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells.

Warmflash A / Sorre B / Etoc F / Siggia ED / Brivanlou AH.

keywords:

hesc, assay, stem cells, developmental biology, maturation, differentiation, gastrulation, spacial patterning

Nat Methods. 2014 Jun 29. doi: 10.1038/nmeth.3016

A Novel Organelle Map Framework for High-Content Cell Morphology Analysis in High Throughput

Schauer K / Grossier J-P / Duong T

keywords:

organelle map, high content screening, high throughput screening

J Biomol Screen. 2013; Aug 16 [epub ahead of print].

A review of microfabrication and hydrogel engineering for micro-organs on chips

Verhulsel M / Vignes M / Descroix S / Malaquin L / Vignjevic DM / Viovy J-L

keywords:

tissue engineering, 3d cell culture

Biomaterials. 2013; Dec 04 [epub ahead of print].

Accumulation of Glucosylceramide in the Absence of the Beta-Glucosidase GBA2 Alters Cytoskeletal Dynamics

Raju D / Schonauer S / Hamzeh H / Flynn KC / Bradke F / Vom Dorp K / Dörmann P / Yildiz Y / Trötschel C / Poetsch A / Breiden B / Sandhoff K / Körschen HG / Wachten D.

keywords:

gba2, glccer, cytoskeleton, sertoli, filopodia, lamellipodia, signaling

PLoS Genet. 2015 Mar 24;11(3):e1005063. doi: 10.1371/journal.pgen.1005063. eCollection 2015.

Age-associated reduction of cellular spreading/mechanical force up-regulates matrix metalloproteinase-1 expression and collagen fibril fragmentation via c-Jun/AP-1 in human dermal fibroblasts

Qin Z / Voorhees JJ / Fisher GJ / Quan T.

keywords:

aging; c-jun/ap-1; extracellular matrix microenvironment; fibroblast; mechanical force; mmp-1.

Aging Cell. 2014 Dec;13(6):1028-37. doi: 10.1111/acel.12265. Epub 2014 Sep 9.

AmotL2 links VE-cadherin to contractile actin fibres necessary for aortic lumen expansion

Hultin S / Zheng Y / Mojallal M / Vertuani S / Gentili C / Balland M / Milloud R / Belting HG / Affolter M / Helker CS / Adams RH / Herzog W / Uhlen P / Majumdar A / Holmgren L.

keywords:

contractility, cadherin, junction, cytoskeleton, aort, lumen, cell shape

Nat Commun. 2014 May 7;5:3743. doi: 10.1038/ncomms4743.

An in vitro one-dimensional assay to study growth factor-regulated tumor cell-macrophage interaction.

Sharma VP / Beaty BT / Cox D / Condeelis JS / Eddy RJ.

keywords:

cell motility, tumor, macrophage

Methods Mol Biol. 2014;1172:115-23. doi: 10.1007/978-1-4939-0928-5_10.

Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity

Théry M / Racine V / Piel M

keywords:

cell polarity, extracellular matrix, morphogenesis, cell normalization, organelle positioning

Proc. Natl. Acad. Sci. U.S.A. 2006;103(52):19771–19776.

Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells

Kremb S / Helfer M / Kraus B / Wolff H / Wild C / Schneider M / Voolstra CR / Brack-Werner R.

keywords:

virus, hiv, infection, virus entry, actin organization

PLoS One. 2014 Aug 21;9(8):e103895. doi: 10.1371/journal.pone.0103895. eCollection 2014.

Arl8 and SKIP act together to link lysosomes to kinesin-1

Rosa-Ferreira C / Munro S

keywords:

lysosomes, kinesin

Dev. Cell. 2011;21(6):1171–1178.

Assessing Kinetics from Fixed Cells Reveals Activation of the Mitotic Entry Network at the S/G2 Transition

Karen Akopyan / Helena Silva Cascales / Elvira Hukasova / Adrian T. Saurin / Erik Müllers / Himjyot Jaiswal / Danielle A.A. Hollman / Geert J.P.L. Kops / René H. Medema / Arne Lindqvist

keywords:

cell cycle, cell division, mitosis

Molecular Cell - 27 February 2014

Carminomycin I is an apoptosis inducer that targets the Golgi complex in clear cell renal carcinoma cells

Woldemichael GM / Turbyville TJ / Linehan WM / McMahon JB

keywords:

high-throughput screening assays, clear cell renal cell carcinoma (ccrcc), von hippel-lindau (vhl) tumor suppressor gene, golgi sequestration, p-gp-mediated localization of anticancer compounds, disk and l shaped fibronectin micropattern

 Cancer Res. 2011;71(1):134–142.

CD81 regulates cell migration through its association with Rac GTPase

Tejera E / Rocha-Perugini V / López-Martín S

keywords:

cell migration, tetraspanin, rho gtpases; focal adhesions

Mol. Biol. Cell. 2012; Dec 05 [epub ahead of print].

Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis

Rodrigues-Fraticelli AE / Auzan M / Alonso MA / Bornens M / Martin-Belmonte F

keywords:

morphogenesis, cell normalization

J Cell Biol. 2012;198(6):1011-1023.

Cell distribution of stress fibres in response to the geometry of the adhesive environment

Théry M / Pépin A / Dressaire E / Chen Y / Bornens M

keywords:

pattern geometry, apices of cell shape, stress fibres, focal adhesions, tractional forces, cell shape, cell surface, cell adhesion, cell line

Cell Motil. Cytoskeleton. 2006;63(6):341–355

Cell shape and contractility regulate ciliogenesis in cell cycle-arrested cells

Pitaval A / Tseng Q / Bornens M / Théry M

keywords:

primary cilium, cilia, cell shape, cell polarity

J. Cell Biol. 2010;191(2):303–312.

Cell-intrinsic adaptation of lipid composition to local crowding drives social behaviour.

Frechin M / Stoeger T / Daetwyler S / Gehin C / Battich N / Damm EM / Stergiou L / Riezman H / Pelkmans L

keywords:

signaling, mechanotransduction, fak, collective behavior, membrane composition

Nature. 2015 May 25. doi: 10.1038/nature14429.